Objective: Aim of the study is to observe the effect of the Hypopressive exercise on peak expiratory flow rate and breath holding time. Method: This is an observational study with young females aged between 18-22 years, with no musculoskeletal or systemic conditions. We analyzed effect of HE in sitting, on PEFR and BHT to establish relation of HE with the lung functions. HE was performed for one month by the subject’s daily 3 sets with 8 repetitions each. Results: Significant increase in values of peak expiratory flow rate and breath holding time of the subjects, who performed hypopressive exercise for a month, was observed when compared with the values measured before performing hypopressive exercise. Conclusion: The results suggested that HE can have positive influence over the PEFR and BHT, when performed for one month daily.