Purpose: The purpose of the study was to compare the difference in calf and groin muscle flexibility between gymnasts and yogis. Method: A total of 60 boys (aged 10-14 years) were selected for the present study which had an equal no. of subjects from gymnastics and yoga. All the subjects had participated in state-level championships. The calf muscle flexibility and groin muscle flexibility were compared between these two groups. To measure the calf muscle flexibility and the groin muscle flexibility of the subjects, the calf muscle flexibility test, and groin muscle flexibility test was conducted. In the statistical procedure, mean and standard deviation (S.D.) were used. To analyze the significant difference, a statistical t-test was applied in SPSS 20. Result: From the present study, it was found that the mean and S.D. of the calf muscle flexibility between gymnasts and yogis were 15.22 & 16.00 and ±3.67 & ±2.57 respectively, and the mean and S.D. of the groin flexibility between gymnasts and yogis were 2.47 & 2.98 and ±2.83 & ±3.46 respectively. The t-value for calf and groin flexibility were found 0.430 and 0.535 respectively. Conclusion: There was no significant difference found in calf muscle flexibility and groin muscle flexibility between state-level gymnasts and yogis.