The purpose of the study was to determine reliability and validity of constructed karate agility test which is specific for kumite match. The research group of 8 karatekas were from local karate clubs in Nitra, Slovakia and were in high fitness level as members of Slovak National Team participated in kumite on average M = 8 years, SD = 2.27 years. The average age of the group was M = 17.87 years, SD = 0.83, average height M = 171 cm, SD = 6.37 and average weight M = 67.12, SD = 10.52. We monitored participants in karate agility test (n = 8). The decisive criterium was time and also the correctness of performing exercises at individual posts, specially kumite techniques, which were judged by karate experts - qualified referees. The criterion was the time for which the proband completed the entire track. Differences between variables were assessed by paired t – test and interactions between the variables by Pearson correlation coefficient “r”. We evaluated the significance of differences and relationships at the 5% and 1% level of significance (p - value). We evaluated the internal realiability of the test by ICC coefficient and Cronbach´s Alpha. The most positive interactions are found between the measurement no. 3 and the measurement no. 4 (r = .981, p <.01). To determine reliability of agility test we used intraclass correlation coeficient (ICC) with good reliability (0.8233) and Cronbach´s Alpha with excellent internal consistency (0.9610). We considered the construction of karate agility test in kumite reliable and valid. The construction of karate agility test in kumite has been fit and proper to be used to measure the karate agility in kumite category.