Background: Hamstring strain injury is a very common injury in sports like basket ball, hockey, football and athletes. In hamstring strain injuries due to change in muscle length and strength will leads to altered recruitment pattern of lumbo-pelvic muscles activation. The present study is about the effect of activity pattern of lumbo-pelvic muscles in plyometric exercises and eccentric hamstring exercises during prone hip extension test in hamstring strain injuries. Objective: To compare the activity pattern of the ipsilateral erector spinae, contralateral erector spinae, gluteus maximus, medial and lateral hamstring muscles in hamstring strain injuries in plyometric exercises and eccentric hamstring exercises during prone hip extension test. Study Design: Experimental study. Procedure: Thirty players with acute hamstring strain injury were conveniently divided in to two groups: Group A (plyometric group) and Group B (eccentric group) for 8 weeks exercise program. The outcome measures are through EMG study. Results: The findings showed that there is a significant difference between both the groups at p value less than 0.005. Conclusion: Eccentric hamstring exercises showed statistically significant improvement in activity pattern of lumbo-pelvic muscles than Plyometric exercises.