Purpose of the present study was to analyse the action of running with shoes and without shoes in respect of some selected kinematic parameters. Fifteen male athletes (sprinters) and fifteen male non-athletes were selected as subject. The selected kinematic parameters were (I) Stride Length (II) Stride Frequency (III) Horizontal Velocity (IV) Stride Time (V) Flight Time (VI) Contact Time (VII) Upper Body Inclination (VIII) Push Leg Inclination (IX) Swing Leg Knee Angle (X) Push Leg Knee Angle (XI) Front Arm Elbow Angle (XII) Back Arm Elbow Angle. The movement of running was recorded by a video camera and the parameters were analyzed by freeze frame technique. To findout the difference on selected kinematic parameter between with shoes and without shoes of athlete group and non-athlete the t-test was conducted and the significant level was set at p<0.05 level. Results revealed that there were statistically significant difference between running with shoes and without shoes in contact time and horizontal velocity for Athlete group but with shoes provide over all better performance for athlete group. For Non Athlete group no significant differences were found in selected parameters.