The primitive man by the very nature of his daily life activities built a strong physique superior to that of a civilized man. In ancient times people lived in the natural environment which forced them to work hard for their live-hood. They had to struggle and fight for their existence. General Physical fitness is the capacity of the body to perform work, to resist disease and infections and to resist physical stresses imposed by such things as heat, cold, atmospheric pressure, changes at high altitude or under water and the force of jolts and vibrations. The purpose of this study was to find out the effect of walking and running on muscular endurance. The subjects were sixty middle aged men from in and around Trirunelveli city were selected as subjects at random and their age was between 35 and 45 years. The subjects were divided into three groups namely walking training group, running training group and control group. Each group consisted of 20 subjects. The duration of the training was twelve weeks in Sankar Polytechnic College, Tirunelveli under the supervision of researcher. The subjects were selected from Tirunelveli city, the study was formulated as a random group design. The score were compared by using (ANCOVA) The level of significant chosen was 0.01level and 0.05 level.