The purpose of this study was find out the effect of specific training on selected physical fitness variables of college level football players. To achieve the purpose (n=26) twenty-six college level football boys from St.Micheale’s College Cherthal, Keral. Their age are ranged from 18 to 25 years. Single group design (experimental group) was used in this study. The following criterion variables were selected for the study namely physical fitness variables are speed, leg explosive power, abdominal strength, flexibility and agility. The training period consists of six weeks Monday to Saturday (except Sunday). The data were collected from before and after the six week of training. The collected data were statistically analyzed by using paired‘t’ test. The level of significance fixed at 0.05 level. The results shows that physical fitness variables are significantly improved due to specific training program on college level football players.