For the purpose of the study, 30 male kho-kho players were randomly selected from University. Out of 30 players, 15 were from Kayani University and 15 from North Bengal University. All of them represented university level tournaments. The age limit of the players ranged between 19-28 years. To find out the flexibility- sit & reach test and strength endurance for sit-ups test were conducted. All statistical calculation was done by standard procedure. Test were analyzed by‘t’ test and significant was set at 0.05 level of confidence. Investigator observed the significant different in flexibility between Kalyani University and North Bengal University Kho-Kho players. The result shows that North Bengal University Kho-Kho players were having more flexibility than Kalyani University Kho- Kho players.