Background: The work of dairy often involves lifting heavy object, moving and carrying equipment, and awkward working posture, all are risk factor of low back injuries and other musculoskeletal problems. The purpose of the study was to identify the prevalence of work-related musculoskeletal disorders among dairy workers in Surat. Objectives: To identify the prevalence and risk factors of work-related musculoskeletal disorders in dairy worker. Methodology: This is an observational study including 50 subjects, both male and female of dairy workers from production and packing department and age between 20-45 years with work experience more than one year in the milk dairy. The subject with previous history of trauma, musculoskeletal problem before joining the dairy, and not willing to participate in study were excluded from study. The study setting was production and packing department of Sumul dairy Surat. Standardized Nordic questionnaire was used to find the prevalence of work-related musculoskeletal disorders among dairy workers. Statistical Analysis: Descriptive analysis of prevalence of work-related musculoskeletal disorders was done by using Microsoft excel 2013. Result: The most common work-related musculoskeletal problem was lower back pain (32%), followed by knee pain (18%), upper back pain (18%), ankle pain (8%), neck pain (4%), wrist pain (4%), shoulder pain (2%). Highest prevalence of low back pain was in age group around 28-35 years. Conclusion: Low back pain is commonest condition affecting dairy workers followed by knee pain, upper back pain, ankle and neck pain respectively, affecting most of the dairy workers in Surat.